A total of 99 spider species from 23 families and two harvestmen species were found during the zoological survey at several sites in Podyjí National Park during 2020. Of them, thirty species are listed in the Red List of Spiders of the Czech Republic. Four new species for Podyjí NP were recorded – Canariphantes nanus (Kulczyński, 1898), Micaria dives (Lucas, 1846), Erigonoplus jarmilae (Miller, 1943) and Ipa keyserlingi (Ausserer, 1867). Other rare species include Alopecosa sulzeri (Pavesi, 1873), Amaurobius jugorum L. Koch, 1868, Arctosa figurata (Simon, 1876), Attulus saltator (O. Pickard-Cambridge, 1868), Berlandina cinerea (Menge, 1872), Callilepis schuszteri (Herman, 1879), Drassyllus villicus (Thorell, 1875), Echemus angustifrons (Westring, 1861), Eresus kollari Rossi, 1846, Evarcha laetabunda (C. L. Koch, 1846), Gnaphosa bicolor (Hahn, 1833), Gnaphosa lugubris (C. L. Koch, 1839), Gnaphosa opaca Herman, 1879, Haplodrassus dalmatensis (L. Koch, 1866), Lasaeola coracina (C. L. Koch, 1837), Minicia marginella (Wider, 1834), Pardosa bifasciata (C. L. Koch, 1834), Phrurolithus pullatus Kulczyński, 1897,
Psammitis ninnii (Thorell, 1872), Pseudeuophrys obsoleta (Simon, 1868), Simitidion simile (C. L. Koch, 1836), Thanatus arenarius L. Koch, 1872, Thomisus onustus Walckenaer, 1805, Trichopterna cito (O. Pickard-Cambridge, 1873), Zelotes erebeus (Thorell, 1871), Zora manicata Simon, 1878 and the harvestman Egaenus convexus (C. L. Koch, 1835).