A total of 2906 specimens of spiders were collected in the mountain valley of the Chomutovka brook in the Krušné hory Mts. between years 2000 and 2020. A total of 336 species were found. According to their abundance and degree of threat, 86 of them belong to significant and rare species. Most significant species are: Achaeridion conigerum, Brommella falcigera, Clubiona corticalis, Dipoena erythropus, Ero tuberculata, Gonatium hilare, Neon levis, Nusoncus nasutus, Pardosa sordidata, Phycosoma inornatum, Talavera thorelli a Theridion boesenbergi.