The investigation was carried out in Northwest Anatolia in the period 2006–2011. From 24 localities, 14 wolf spider species in six genera were reported. The species represent lowland as well as mountain spider fauna of the Palaearctic region. In this study, Alopecosa farinosa, Aulonia albimana, Pardosa consimilis, Pardosa hortensis and Pardosa monticola are the first records for Marmara region in Turkey. Trabea paradoxa is the first record for Anatolia.