Data on the spider fauna from the forest ecosystems of the Děvín National Nature Reserve (South Moravia, Czech Republic) are presented. The research was carried out on 15 randomly chosen research plots in oak-hornbeam forest stands within different management regimes. Spider sampling was done by pitfall trapping from May to October 2016. Overall, 3683 adult spiders belonging to 22 families, 70 genera and 116 species were collected. The families Linyphiidae, Lycosidae, Gnaphosidae and Thomisidae exhibited high species richness. The most abundant species were
Pardosa lugubris (Walckenaer, 1802) and
Trochosa terricola Thorell, 1856. Faunistically remarkable species were
Atypus piceus (Sulzer, 1776),
Drassyllus villicus (Thorell, 1875),
Gnaphosa montana (L. Koch, 1866),
Panamomops affinis Miller &
Kratochvíl, 1939 and
Walckenaeria monoceros (Wider, 1834). The record of
Gnaphosa montana is one of the first accounts of this psychrophilic spider from European lowlands. Of the identified species, 23 are listed in the Red List of Threatened Species in the Czech Republic (EN – 1 species, VU – 12 species, LC – 10 species).